Ace my homework – Write
Objective, Background, method, result and conclusion (ALL IN PAST TENS )
ANSWER THE Questions.
SEE THE RESULTS IN EXCEL SHEET
a table of the data from the growth experiment
Time in mins
count date from each group.
average data
actual dilution (remember the 0.1 ml added to the plate is another 10 fold dilution)
Cfu/ml (represent with one digit to the left of the decimal point and two to the right then the exponent)
Absorbance at 600nm
Calculated CFU using the Aligent website
GraphTitleY axisX axisE coli Growth CurveAbsorbance (600nm)Time (mins)E. coli Growth CurveLog bacterial numbers (CFU MM/ml) Aligent)TimeE. coli Growth CurveLog bacterial numbers (CFU MM/ml)TimeE coli Standard CurveAbsorbance (600nm)Cell numbers (MM/ml) for both Aligent and your CFU data
Questions
Look at the growth curves using Aligent CFU and your CFU: are they similar or different. If they are different why is that..
Look at the aligent plate count vs absorbance graph – are they similar are they different. If they are different why do you think that
Look at the aligent and your cfu data vs time – calculate the maximum time of doubling
